Search results for " Acquired"

showing 10 items of 48 documents

Purification, crystallization and preliminary X-ray studies of sylvaticin, an elicitin-like protein from Pythium sylvaticum.

2003

Sylvaticin belongs to the elicitin family. These 10 kDa oomycetous proteins induce a hypersensitive response in plants, including necrosis and cell death, but subsequently leading to a non-specific systemic acquired resistance (SAR) against other pathogens. Sylvaticin has been crystallized using PEG 2000 MME as a precipitant agent in the presence of nickel chloride. The crystals belong to space group C2, with unit-cell parameters a = 99.29, b = 25.67, c = 67.45 A, beta = 99.66 degrees. Diffraction data were recorded to 2.1 A resolution at a synchrotron-radiation source.

Hypersensitive responseStereochemistryProtein ConformationPythiumBiologyCrystallography X-Raylaw.inventionPolyethylene GlycolsProtein structureStructural BiologylawNickelPEG ratioCrystallizationFuransAlgal ProteinsX-rayProteinsElicitinGeneral Medicinebiology.organism_classificationCrystallographySolventsPythium sylvaticumSystemic acquired resistanceSynchrotronsActa crystallographica. Section D, Biological crystallography
researchProduct

Free Radicals Mediate Systemic Acquired Resistance

2014

Summary: Systemic acquired resistance (SAR) is a form of resistance that protects plants against a broad spectrum of secondary infections. However, exploiting SAR for the protection of agriculturally important plants warrants a thorough investigation of the mutual interrelationships among the various signals that mediate SAR. Here, we show that nitric oxide (NO) and reactive oxygen species (ROS) serve as inducers of SAR in a concentration-dependent manner. Thus, genetic mutations that either inhibit NO/ROS production or increase NO accumulation (e.g., a mutation in S-nitrosoglutathione reductase [GSNOR]) abrogate SAR. Different ROS function additively to generate the fatty-acid-derived azel…

0106 biological sciences[SDV]Life Sciences [q-bio]ArabidopsisPseudomonas syringaeReductasemedicine.disease_cause01 natural scienceschemistry.chemical_compoundcuticle formationInducerDicarboxylic Acidsskin and connective tissue diseaseslcsh:QH301-705.5chemistry.chemical_classification0303 health sciencesMutationsalicyclic-acidCell biologydefenseGlutathione ReductaseBiochemistryGlycerophosphates[SDE]Environmental Sciencesplant immunitySystemic acquired resistances-nitrosoglutathioneSecondary infectionnitric-oxidearabidopsis-thalianaBiologyNitric OxideGeneral Biochemistry Genetics and Molecular BiologyNitric oxide03 medical and health sciencesmedicine[SDV.BV]Life Sciences [q-bio]/Vegetal Biology030304 developmental biologyReactive oxygen speciesArabidopsis Proteinsfungicell-deathbody regionschemistrylcsh:Biology (General)azelaic-acidresponsesNitric Oxide SynthaseReactive Oxygen SpeciesFunction (biology)010606 plant biology & botanynitric-oxide;plant immunity;arabidopsis-thaliana;s-nitrosoglutathione;cuticle formation;salicyclic-acid;azelaic-acid;cell-death;responses;defenseCell Reports
researchProduct

Community-acquired respiratory virus lower respiratory tract disease in allogeneic stem cell transplantation recipient: Risk factors and mortality fr…

2018

Abstract Risk factors (RFs) and mortality data of community‐acquired respiratory virus (CARVs) lower respiratory tract disease (LRTD) with concurrent pulmonary co‐infections in the setting of allogeneic hematopoietic stem cell transplantation (allo‐HSCT) is scarce. From January 2011 to December 2017, we retrospectively compared the outcome of allo‐HSCT recipients diagnosed of CARVs LRTD mono‐infection (n = 52, group 1), to those with viral, bacterial, or fungal pulmonary CARVs LRTD co‐infections (n = 15, group 2; n = 20, group 3, and n = 11, group 4, respectively), and with those having bacterial pneumonia mono‐infection (n = 19, group 5). Overall survival (OS) at day 60 after bronchoalveol…

Male0301 basic medicinemedicine.medical_treatmentcommunity acquired respiratory virusHematopoietic stem cell transplantationBronchoalveolar LavageGastroenterology0302 clinical medicineRisk Factorsrespiratory virus co‐infectionsLungRespiratory Tract Infectionsmedicine.diagnostic_testRespiratory tract infectionsCoinfectionHematopoietic Stem Cell TransplantationMiddle AgedCommunity-Acquired InfectionsInfectious Diseasesmedicine.anatomical_structureVirusesvirus-bacterial mixed infectionsRespiratory virusFemaleOriginal Articlerespiratory virus co-infectionsBronchoalveolar Lavage FluidAdultmedicine.medical_specialtyvirus‐bacterial mixed infections030106 microbiologyContext (language use)CMV DNAemiaAntiviral Agents03 medical and health sciencesInternal medicinemedicineHumansTransplantation Homologousallogeneic hematopoietic stem cell transplantationAgedRetrospective StudiesTransplantationLungBacteriabusiness.industryFungiBacterial pneumoniaOriginal Articlesmedicine.diseaseTransplantationBronchoalveolar lavagebusinessimmunodeficiency score index030215 immunology
researchProduct

Physiological and Molecular Characteristics of Elicitin-Induced Systemic Acquired Resistance in Tobacco

1996

Elicitins are low molecular weight proteins secreted by all Phytophthora species analyzed so far. Application of the purified proteins to tobacco Nicotiana tabacum leads to the induction of resistance to subsequent inoculations with the black shank-causing agent, Phytophthora parasitica var nicotianae. In this paper, we describe the systemic characteristics of elicitin-induced acquired resistance in tobacco. Elicitin application is followed by the rapid translocation of the protein in the plant. The basic elicitin, cryptogein, induces necrosis formation in the leaves, which results from accumulation of the protein in these organs. Necrosis does not seem to be essential for the establishment…

0106 biological sciencesPhysiologyNicotiana tabacumPlant Science01 natural sciences[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health sciences[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants geneticsGene expressionBotanyGeneticsGeneComputingMilieux_MISCELLANEOUS030304 developmental biology0303 health sciencesbiologyINDUCTIONfungiElicitinPhytophthora nicotianaebiology.organism_classificationCell biologyPhytophthoraRESISTANCESystemic acquired resistanceSolanaceaeResearch Article010606 plant biology & botanyPlant Physiology
researchProduct

International prevalence and risk factors evaluation for drug-resistant Streptococcus pneumoniae pneumonia

2019

Objective: Streptococcus pneumoniae is the most frequent bacterial pathogen isolated in subjects with Community-acquired pneumonia (CAP) worldwide. Limited data are available regarding the current global burden and risk factors associated with drug-resistant Streptococcus pneumoniae (DRSP) in CAP subjects. We assessed the multinational prevalence and risk factors for DRSP-CAP in a multinational point-prevalence study. Design: The prevalence of DRSP-CAP was assessed by identification of DRSP in blood or respiratory samples among adults hospitalized with CAP in 54 countries. Prevalence and risk factors were compared among subjects that had microbiological testing and antibiotic susceptibility…

0301 basic medicineMaleStreptococcus pneumoniaantibiotic resistanceInternationalitysputum examinationbronchiectasisvery elderlyAntibioticsPrevalenceDrug resistancemedicine.disease_causeLogistic regressionGlobal HealthCommunity-Acquired Infections/epidemiologylung lavage0302 clinical medicineCommunity-acquired pneumoniaCost of IllnessRisk FactorsPrevalencedrug resistant Streptococcus pneumoniae pneumonia030212 general & internal medicineMicrobial drug resistantAged 80 and overadultinternational cooperationdrug effectMiddle Agedinfluenza vaccinationAnti-Bacterial Agentsantiinfective agentEuropeCommunity-Acquired InfectionsHospitalizationGlobal burden of diseaseStreptococcus pneumoniaeInfectious Diseasesrisk factorbacterium identificationFemalecommunity acquired infectioninfluenzaliver diseasepneumococcal vaccinationPneumococcal infectionhospitalizationmedicine.drugMicrobiology (medical)medicine.medical_specialtyAsiamedicine.drug_class030106 microbiologySettore MED/10 - Malattie Dell'Apparato RespiratorioArticleAnti-Bacterial Agents/pharmacology03 medical and health sciencesInternal medicineStreptococcus pneumoniaeDrug Resistance BacterialPneumonia Pneumococcal/epidemiologymedicineHumanscontrolled studyhumantetracyclineHospitalization/statistics & numerical dataAgedlevofloxacinnonhumanbusiness.industrydisease associationmicrobiologycommunity acquired pneumoniamacrolidePneumoniaasthmaSouth AmericaPneumonia Pneumococcalvaccinationmedicine.diseasemajor clinical studyantibiotic sensitivitypenicillin derivativePenicillinStreptococcus pneumoniae/drug effectsPneumoniablood examinationAfricaNorth Americamicrobiological examinationbusinessGlobal burden of disease; Microbial drug resistant; Pneumococcal infection; Pneumonia
researchProduct

Assessment of immunodeficiency scoring index performance in enterovirus/rhinovirus respiratory infection after allogeneic hematopoietic stem cell tra…

2020

BACKGROUND: Enterovirus/rhinoviruses (EvRh) are the most common cause of respiratory virus infections in recipients of allogeneic stem cell transplantation (allo-HSCT).; OBJECTIVE: We sought to analyze the value of the immunodeficiency scoring index (ISI) in predicting lower respiratory tract disease (LRTD) progression and mortality in a prospective cohort of consecutive adult (> 16 years) allo-HSCT recipients with EvRh infection from December 1 2013 to December 1 2019 at two Spanish transplant centers.; RESULTS: We included 234 allo-HSCT recipients with 383 EvRh episodes. Out of 383 EvRh episodes, 98 (25%) had LRTD. Multivariate logistic regression analysis identified three independent fac…

AdultMalemedicine.medical_specialtyAdolescentRhinovirusmedicine.medical_treatmentHematopoietic stem cell transplantationcommunity acquired respiratory virus030230 surgerymedicine.disease_cause03 medical and health sciencesYoung Adult0302 clinical medicineInternal medicineallogeneic stem celltrasplantationmedicineHumansTransplantation HomologousProspective StudiesProspective cohort studyRespiratory Tract InfectionsImmunodeficiencyimmunodeficiency scoring indexAgedRetrospective StudiesTransplantationPicornaviridae InfectionsRespiratory tract infectionsbusiness.industryHematopoietic Stem Cell TransplantationImmunologic Deficiency SyndromesRespiratory infectionMiddle Agedmedicine.diseaseTransplantationInfectious DiseasesrhinovirusROC CurveSpainMultivariate AnalysisRespiratory virus030211 gastroenterology & hepatologyFemaleRhinovirusbusiness
researchProduct

Amelanotic conjunctival melanoma

2006

Conjunctival melanoma is a rare condition of the eye pigment predominantly affecting white adults. We describe a 32-year-old white man with an amelanotic malignant melanoma of the conjunctiva that is not associated with primary acquired melanosis (PAM) or melanocytic nevus. The patient presented with a 2-year history of nonpigmented vascularized nodules of the right eye. Results of hematoxylin and eosin (H and E) staining of the lesion showed an invasive nodule with vertical spreading, invasion of the substantia propria corneae, and ulceration. S100 protein was expressed in the cells of the invasive nodule. HMB45 protein was highly positive in the melanoma cells. The de novo amelanotic mali…

AdultMaleConjunctival melanomaprimary acquired melanosis (PAM).HumansConjunctival NeoplasmsMelanoma AmelanoticConjunctivaImmunohistochemistryamelanotic malignant melanoma
researchProduct

ICU-acquired infections: It is not only about the number of patients per room

2016

LETTER TO EDITOR

Cross infectionmedicine.medical_specialtyCross Infectionbusiness.industryIntensive Care Unit030208 emergency & critical care medicineCritical Care and Intensive Care Medicine03 medical and health sciencesIntensive Care Units0302 clinical medicineText miningEmergency medicineMedicineHumans030212 general & internal medicineMED/41 - ANESTESIOLOGIAbusinessICU acquired infection sepsis critical careHuman
researchProduct

Nasal airway in cleft-palate patients: acoustic rhinometric data

1997

The objective of this study was to investigate an instrumental assessment technique for acquiring reproducible, metric data on the nasal airway in cleft-palate associated nasal dysplasia. A consecutive sample of 23 unilateral, 17 bilateral CLP patients and 15 controls with subjective normal nasal patency from a cleft-palate rehabilitation centre were studied. A series of transnasal acoustic measurements (pressure wave: 55 dB for 2 ms) of nasal volume were performed before and after topical decongestion with 2 x 0.3 mg of xylometazoline. A standardized regimen of acoustic parameters of the nasal valve and the adjacent segment of the nasal cavity were calculated. The cleft side yielded a sign…

AdultNasal cavitymedicine.medical_specialtyAdolescentCleft Lipmedicine.medical_treatmentNoseRhinoplastyXylometazolineAcoustic rhinometryotorhinolaryngologic diseasesmedicineHumansLongitudinal StudiesChildNosemedicine.diagnostic_testbusiness.industryImidazolesNose Deformities AcquiredReproducibility of ResultsAcousticsHypertrophyrespiratory systemRhinoplastySurgeryCleft PalatePosterior segment of eyeballNasal DecongestantsNasal MucosaSoundmedicine.anatomical_structureOtorhinolaryngologySurgeryNasal CavityNasal ObstructionOral SurgeryRhinomanometryAirwaybusinessFollow-Up Studiesmedicine.drugJournal of Cranio-Maxillofacial Surgery
researchProduct

Proteinaceous and oligosaccharidic elicitors induce different calcium signatures in the nucleus of tobacco cells.

2005

We previously reported elevated cytosolic calcium levels in tobacco cells in response to elicitors [D. Lecourieux, C. Mazars, N. Pauly, R. Ranjeva, A. Pugin, Analysis and effects of cytosolic free calcium elevations in response to elicitors in Nicotiana plumbaginifolia cells, Plant Cell 14 (2002) 2627-2641]. These data suggested that in response to elicitors, Ca2+, as a second messenger, was involved in both systemic acquired resistance (RSA) and/or hypersensitive response (HR) depending on calcium signature. Here, we used transformed tobacco cells with apoaequorin expressed in the nucleus to monitor changes in free nuclear calcium concentrations ([Ca2+](nuc)) in response to elicitors. Two …

Hypersensitive responsePhysiologyAequorinMutant Chimeric Proteinschemistry.chemical_elementOligosaccharidesCalciumTobaccoCalcium SignalingPhosphorylationMolecular BiologyCells CulturedCalcium signalingPlant ProteinsCell Nucleusbiologyfood and beveragesCell BiologyElicitorCytosolchemistryBiochemistrySecond messenger systemGene Targetingbiology.proteinSystemic acquired resistanceCell calcium
researchProduct